Protein Purification Reagents and Kits
Biochain Institute Inc CNMCS Compartmental Protein Extraction Kit: Buffer M, 6 ml/PK
One of the many challenges in the field of proteomics is separation of complex protein mixtures for quantitative and differential localization analysis. To directly address this issue, BioChain is pleased to offer the CNMCs Compartmental Protein Extraction Kit. This kit is designed for rapid and efficient sequential isolation of cytoplasmic, nuclear, membrane, and cytoskeletal proteins, all from the same tissue or cell samples. The system utilizes our proprietary buffers that have been optimized to prepare the highest quality compartmental protein extractions. The kit also includes our proprietary protease inhibitor cocktail to prevent protein degradation. In conjunction, these components result in protein extracts that may be used for downstream applications such as SDS-PAGE, isoelectric focusing and 2-D gel electrophoresis, Western blotting, gel mobility shift analysis, and other protein assays.
Hampton Research HR2-932-D6/Grid Screen Sodium Chloride #D6/Grid Screen Salt HT (H12), 185 ml - 0.1 M BICINE pH 9.0, 4.0 M Sodium chloride
HR2-932-D6/Grid Screen Sodium Chloride #D6/Grid Screen Salt HT (H12), 185 ml - 0.1 M BICINE pH 9.0, 4.0 M Sodium chloride. Please note items from this supplier are non-returnable.
Biochain Institute Inc Membrane Protein - Human Tumor Tissue: Skin, 0.1 mg/PK
Tissue membrane protein is prepared from whole tissue homogenates and presents a consistent pattern on SDS-PAGE analysis. The protein is stored in a buffer with protease inhibitor cocktail.
Biochain Institute Inc Membrane Protein - Human Adult Normal Tissue: Kidney, 0.1 mg/PK
Tissue membrane protein is prepared from whole tissue homogenates and presents a consistent pattern on SDS-PAGE analysis. The protein is stored in a buffer with protease inhibitor cocktail.
Enzo Life Sciences EPIXTRACT Nuclear Protein Isolation Kit II (1x100Reactions)
Rapid, reliable method to isolate nucleic acid-free nuclear proteins in mammalian cells and tissues. EPIXTRACT Nuclear Protein Isolation Kit II provides a simple and reliable method to isolate nucleic acid-free nuclear proteins. Extracts can be used for a variety of downstream applications including western blotting, protein-DNA binding assays, nuclear enzyme assays and others requiring nucleic acid-free nuclear proteins. Assay Time: 1 hour. Contents: Pre-extraction Buffer, Extraction Buffer, Extraction pre-cleaner, Extraction cleaner, Protease Inhibitor Cocktail, DTT
Hampton Research HR2-998-43/PEG/Ion 2 Screen #43 (H07), 185 ml - 0.01 M Magnesium chloride hexahydrate, 0.005 M Nickel(II) chloride hexahydrate, 0.1 M HEPES sodium pH 7.0, 15% w/v Polyethylene glycol 3,350
HR2-998-43/PEG/Ion 2 Screen #43 (H07), 185 ml - 0.01 M Magnesium chloride hexahydrate, 0.005 M Nickel(II) chloride hexahydrate, 0.1 M HEPES sodium pH 7.0, 15% w/v Polyethylene glycol 3,350. Please note items from this supplier are non-returnable.
LifeSensors Tandem Ubiquitin Binding Entities (TUBE1) Agarose 1mL
Based on protein domains known to possess an affinity for ubiquitin, Tandem Ubiquitin Binding Entities (TUBEs) have been developed for the isolation and identification of ubiquitinated proteins. TUBEs display up to a 1000-fold increase in affinity for polyubiquitin moieties over the single ubiquitin binding associated domain (UBA). In addition, TUBEs protect polyubiquitinated proteins from deubiquitination and proteasomal degradation, allowing for detection at relatively low abundance. These properties effectively allow TUBEs to “capture” protein in its polyubiqutinated state. TUBEs can be used to isolate, enrich, and identify ubiquitinated proteins from cell and tissue extracts. Agarose coupled TUBEs allow for efficient recovery of polyubiquitinated proteins in a single step. Polyubiquitinated protein can easily be separated from TUBEs for proteomic studies or identification by Western blotting. This product is protected by one or more US or Foreign patents.
Biochain Institute Inc Membrane Protein - Human Adult Normal Tissue: Vagina, 0.1 mg/PK
Tissue membrane protein is prepared from whole tissue homogenates and presents a consistent pattern on SDS-PAGE analysis. The protein is stored in a buffer with protease inhibitor cocktail.
Biochain Institute Inc Membrane Protein - Monkey (Rhesus) Normal Tissue: Brain, 0.1 mg/PK
Tissue membrane protein is prepared from whole tissue homogenates and presents a consistent pattern on SDS-PAGE analysis. The protein is stored in a buffer with protease inhibitor cocktail.
Creative Diagnostics Purified exosomes, Lyophilized, 2 x 100 μg, 50-120 nm, Human Plasma, Reconstitute with 100 μL deionized water
Purified exosomes, Lyophilized, 2 x 100 μg, 50-120 nm, Human Plasma, Reconstitute with 100 μL deionized water
Biotium Cytokeratin 18(DA7), CF594 conjugate, 0.1mg/mL
This MAb reacts with a wide variety of simple epithelia. It does not react with stratified squamous epithelia. It reacts with epithelial tumors of the gastrointestinal tract, lung, breast, pancreas, ovary, and thyroid. Cytokeratin 18, which belongs to the type A (acidic) subfamily of low molecular weight keratins, exists in combination with cytokeratin 8. It was reported that tissues from gastrointestinal tract are positive for both cytokeratin 8 and 18 but do not contain cytokeratin 14. Tissues from gastrointestinal tract, respiratory tract and urogenital tract, as well as endocrine and exocrine tissues and mesothelial cells are positive for cytokeratin 18.Primary antibodies are available purified, or with a selection of fluorescent CF Dyes and other labels. CF Dyes offer exceptional brightness and photostability. Note: Conjugates of blue fluorescent dyes like CF405S and CF405M are not recommended for detecting low abundance targets, because blue dyes have lower flu
Biochain Institute Inc Total Protein Extraction Kit, 1 kit/PK
BioChain's Total Protein Extraction Kit is designed for rapid and efficient isolation of total protein lysates from virtually any tissue or cell sample. The system utilizes our proprietary buffer that has been optimized to prepare the highest quality total protein extractions. The kit also includes our proprietary protease inhibitor cocktail to prevent protein degradation. In conjunction, these two components result in protein extracts that are native and may be used for downstream applications such as SDS-PAGE, isoelectric focusing and 2-D gel electrophoresis, Western blotting, tissue-specific protein expression analysis, immunoprecipitation, protein-protein interaction studies, gel mobility shift analysis, DNA-protein interaction studies, antibody production, and enzymatic activity assays. Our Total Protein Extraction Kit is user-friendly and contains enough reagents for isolating total proteins from up to 5 grams of tissue or 125 million cultured cells. The kit is also scalable.
LifeSensors Tandem Ubiquitin Binding Entities (TUBE2) Biotin 200µg
Based on protein domains known to possess an affinity for ubiquitin, Tandem Ubiquitin Binding Entities (TUBEs) have been developed for the isolation and identification of ubiquitylated proteins. TUBEs display up to a 1000-fold increase in affinity for polyubiquitin moieties over the single ubiquitin binding associated domain (UBA). In addition, TUBEs display a protective effect on polyubiquitinated proteins, allowing for detection at relatively low abundance. These properties effectively “capture” proteins in their polyubiquitylated state. Biotin-TUBEs allow for the detection of polyubiquitin and polyubiquitylated proteins by ligand blotting (“far Western”) without heating the membrane. This reagent is a superior alternative to traditional polyubiquitin immunodetection techniques, such as anti-ubiquitin IgGs.
CELL GUIDANCE SYSTEMS LLC EXO-SPIN 96
Exo-spin™ 96 provides proven size exclusion chromatography (SEC) mini-column (EX01) technology in a 96-well format, allowing up to 96 individual samples to be processed in less than 30 minutes. Sample volumes up to 0.5 ml can be purified. The kit provides flexibility with 8-column strips detachable from the plate, so you can process 8 samples in 8-column strips if neededThe Exo-spin™ 96 well product offers:High-throughput exosome isolation at the benchtop in the standard 96 well format in under 30 minutesPurify up to 0.5 ml of each sampleUp to 99% of free proteins removedOutstanding reproducibility +/- 5% Standard deviation, for consistent exosome purification, time after timeFlexibility to just work with single strips of 8 columns at a timeThe convenience of purified exosomes eluted directly into a 96 well plate ready for high throughput downstream applications
